Biohydrolysis of Banana and Plantain Peels for the Production of Biofuel

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F. I. Jumare
A. M. Magashi
A. B. Rabah
A. M. Sokoto
M. U. Hizbullahi


This study was carried out to assess the potentials of banana and plantain peel as feedstock for biofuel production. Fungi were isolated from spoiled banana, burkutu and spoiled bread using the standard microbiological method. The concentration of reducing sugar of the peels were measured using DNS calorimetry method and biofuel were measured using chromium (VI) reagent by Ultraviolet-Visible Spectrophotometer. Aspergillus niger, Saccharomyces cerevisiae and Mucor racemusus were isolated. A reducing sugar concentration of 59.12 mg/g and 56.62 mg/g was observed for the banana and plantain peels. The highest concentration was found to be 0.35 mg/L for banana peels and 0.10 mg/L for plantain. The IR characterization of the banana and plantain sample revealed an intense strong broad band of alcohol O-H and alkane C-H stretching. The GC-MS result revealed the presence of benzaldehyde in all the biomass while 2,3-butanediol was only detected in the plantain peels biomass. This study showed the potential of banana and plantain peels biomass for biofuel production.

Biofuel, banana peels, plantain peels, hydrolysis, Saccharomyces cerevisiae, mucor racemus.

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How to Cite
Jumare, F. I., Magashi, A. M., Rabah, A. B., Sokoto, A. M., & Hizbullahi, M. U. (2019). Biohydrolysis of Banana and Plantain Peels for the Production of Biofuel. Journal of Energy Research and Reviews, 3(2), 1-11.
Original Research Article


Goldemberg J. In proceeding of the conference on ecological dimensions of Biofuels. Washigton, DC; 2008.

Pimentel D, Patzek TW. Ethanol production using corn. Switch grass and wood; biodiesel production using soybean and sunflower. Nat. Resource Res. 2005;14(1):65-76.

Tchobanoglous G, Theisen H, Vigil S. Integrated solid waste management engineering principles and management issues. MCGraw-Hill, New York. 1993;3–22.

Bassey N. Oil politics: Nigeria’s unacceptable biofuels policy; 2010.

Oniemola PK, Sanusi G. The Nigerian bio-fuel policy and incentives. A need to follow the Brazilian pathway. International Association for Energy Economics. 2009; 56-70.

Devanathan A, Shanmugun T, Balasubraminian, Manivannan S. Cellulase production from coastal mangrove debris. Trends Appl. Sci. Res. J. 2007;2:23-27.

Domsch KH, Grams W. Fungi in agricultural soils, Longman group limited. London. 1970;20-152.

Oyeleke SB, Manga SB. Essential laboratory practical in microbiology. 1st edition. TOBEST publishers, Minna Nigeria. 2008;36-37.

David E, Stephen D, Helen A, Rosemary H, Robyn B. Descriptions of medical fungi. 2nd edition. Australia. 2007;100.

Ekunsaumi T. Laboratory production and assay of Amylase by fungi and Bacteria. Bio-Link Organization, UW-Washington Country. 2006;23-34.

Oyeleke SB, Dauda BE, Oyewole OA, Okoliegbe IN, Ojebode T. Production of bioethanol from cassava and sweet potato peels. Advances in Environ. Biology. 2012;6(1):241-245.

Miller GL. Use of dinitosalicyclic acid reagent for determination of reducing. Analytical Chemistry. 1959;31:426.

Patel SJ, Onkarappa R, Shoba KS. Fungal pretreatment studies on rice husks and Bagasse. Electronic J. of Environ. Agric. and Food Chemistry. 2007;6(4):1921-1926.

Rabah AB, Oyeleke SB, Manga SB, Hassan LG. Microbial pre treatment of rice husk and groundnut shell for bioethanol production. Int. Research J. Microbiol. 2011;2(8):253-258.

Mie-ling W, Youk-meng C, Nan-wei S, Hsiung L. A rapid method for determination of ethanol in alcoholic beverages using capillary gas chromatography. J. food drug Analysis. 2003;11(2):133-140.

Acharya PB, Acharya DK, Modi HA. Optimization for Cellulase production by Aspergillus niger using sawdust as substrate. Afri. J. Biotech. 2008;7(22): 4147–4152.

Essien J, Akpan E, Essien E. Studies on mould growth and biomass production using waste banana peel. Biores. Technol. 2005;96(13):1451–1456.

Yabaya A, Ado SA. Mycelial protein production by Aspergillus niger using banana peels. Sci. World J. 2008;3:9-12.

Vallet C, Said R, Rabiller C, Martin ML. Natural abundance isotopic fractionation in the fermentation reaction: Influence of the nature of the yeast. Bioorganic Chem. 1996;24:319-330.

Elijah AJ, Ojimelukwe PC, Ekong US, Asamudo NU. Effect of Sacoglottis gabonensis and Alstonia boonei on the kinetics of S. cerevisiae isolated from palm wine. Afr. J. Biotech. 2010;9(35):5730-5734.

Auta HS, Oyeleke SB, Ayodele OB, Bala JD, Abioye OP, Ibrahim AD, Ijah UJJ, Manga SB. Characterization of extra-cellular amylase produced by Aspergillus fumigatus isolated from rice husk waste dumpsite. Biosciences. 2012;6(6):155-159.

Laopaiboon P, Thani A, Leelavatcharamas V, Laopaiboon L. Acid hydrolysis of sugarcane Bagasse for lactic acid production. Bioresource Technol. 2010; 101:1036–1043.

Itelima J, Onwuliri F, Onwuliri E, Onyimba I, Oforji S. Bio-ethanol production from banana, plantain and pineapple peels by simultaneous saccharification and fermentation process. Int. J. Environ. Sci. and Devel. 2013;4:2-12.

Jones RP, Greenfield PF. Role of water activity in ethanol fermentation. Biotechnol. and Bioeng. 1986;XXVII:29–40.

Hallsworth JE. Ethanol induced water stress in yeast. J. of Ferm. and Bioeng. 1998;85(2):125-137.

Epstein JL, Vieira M, Aryal B, Vera N, Solis M. Developing biofuel in the teaching laboratory: Ethanol from various sources. J. Chem. Edu. 2010;87(7):708-710.

Cheng SW, Anderson BC. Investigation of ethanol production from municipal primary waste water. Biores. Technol. 1997;59:81-96.

Huang XL, Penner MH. Apparent substrate inhibition of the Trichoderma reesei cellulase system. J. Agric. Food Chem. 1991;39:2096–2100.

Penner MH, Liaw ET. Kinetic consequences of high ratios of substrate to enzyme saccharification systems based on Trichoderma cellulase. In: Himmel ME, Baker JO, Overend RP. (Eds.). Enzymatic conversion of biomass for fuels production. American Chemical Society, Washington, DC. 1994;363–371.

Reddy HK, Srijana M, Madhusudhan RD, Gopal R. Coculture fermentation of banana agro-waste to ethanol by Cellulolytic thermophilic, Clostridium thermocellum CT2. Afri. J. Biotechnol. 2010;9(13):1926-1934.

Wen Z, Liao W, Chen S. Hydrolysis of animal manure lignocellulosic for reducing sugar production. Biores. Technol. 2004;91:31-39.

Mohamed MA, Reddy CA. Direct fermentation of potato starch to ethanol by cocultures of Aspergillus niger and Saccharomyces cerevisiae. Applied and Environ. Microbiol. 1986;1055-1059.

Ocloo FCK, Ayernor GS. Production of alcohol from cassava flour hydrolysate. J. of Brewing and Distilling. 2010;1(2):15-21.

Kordylas JM. Processing and preservation of tropical and subtropical foods. Macmillan Education Limited, Houndmills. 1990;105-107.

Ueda S, Zenin CT, Monteiro DA, Park YK. Production of ethanol from raw cassava starch by a non-conventional fermentation method. In: John Wiley and Sons Inc. Biotechnol. and Bioeng. 1981;23:291-299.

Togarepi E, Mapiye C, Muchanyereyi N, Dzomba P. Optimization of fermentation parameters for ethanol production from Ziziphus mauritiana fruit pulp using Saccharomyces cerevisiae (NA33). Int. J. Biochem. Res. & Review. 2012;2(2):60- 69.

Bodirlau R, Teaca CA, Spiridon I. Chemical modification of beech wood: Effect on thermal stability. Bioresources. 2008;3(3):789-800.

Flickinger MC. Current biological research in conversion of cellulosic carbohydrate into liquid fuels: How far have we come? Biotechnol. Bioeng. 1980;22:27-48.

Ng Chiam Y, Moo-young J, Jinwon L, Min-kyu O. Production of 2,3-butanediol in Saccharomyces cerevisae by insilico aided metabolic engineering. Microbial cell factories. 2012;11:68.