Biogas Yielding Potential of Maize Chaff Inoculated with Cow Rumen and Its Characterization

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S. Chukwuka Iweka
K. C. Owuama


Human Life on earth is driven by energy and with the global challenge on best ways to manage waste, there is need to convert organic waste to bioenergy which will help reduce the rate of environmental pollution and over dependence on conventional source of energy. In this investigation maize chaff were inoculated with cow rumen using different concentration ratios (S/I) of 1:1, 1: 1.55, 1:3.5 for 25, 31 and 37 days Retention Time (RT) as design by Central Composite Face Centered Design to optimize the process and predict the best response. The result obtained shows that the mixture ratio of 0.65 (1:1.55) for 31 days gave the optimum yield while 0.65 mixing ratio for 37 days gave the maximum yield at 0.42L under mesophilic (20°C to 45°C) condition. The Flash point of the cummulative maximum yield was -164°C which is really flammable. The model F-value is 95.03, p-values is < 0.0001 which is less than 0.05 and both values indicate model terms are significant. Lack of Fit F-value of 0.43 implies the fitting effect is good. Its R2 value of 0.9855 is very close to 1 which is good. In addition, the biogas products were characterized by FTIR spectroscopy and Gas chromatography–mass spectrometry (GC-MS). The FTIR analyzes showed the presence of Alcohol and was further proven by 69% methane gotten as indicated by the GC-MS. Thus, the result shows high methane yield, flammability and suitability for maize chaff inoculated with cow rumen for energy production.

Cow rumen, anaerobic digestion, maize chaff, biogas production, optimization, inoculation, bioenergy, characterization.

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How to Cite
Iweka, S. C., & Owuama, K. C. (2020). Biogas Yielding Potential of Maize Chaff Inoculated with Cow Rumen and Its Characterization. Journal of Energy Research and Reviews, 6(3), 34-50.
Original Research Article


Anisiji OE, Chukwuneke JL, Achebe CH, Okolie PC. Integrated process modeling of a thermophilic biogas plant. International Journal of Scientific & Technology Research. 2014;3(7):334–341.

Iweka CS, Owuama KC, Swift ONK. Nigeria beyond oil. Proceedings of 4th International Conference on Engineering Adaptation and Policy Reforms (ICEPAR 2019). 2019;128–136.

Chukwuneke JL, Ewulonu MC, Chukwujike IC, Okolie PC. Physico-chemical analysis of pyrolyzed bio-oil from Swietenia macrophylla (mahogany) wood. Heliyon. 2019;5:e01790.
DOI: 10.1016/j.heliyon.2019.e01790

Onyenobi CS, Chukwuneke LJ, Achebe HC, Okolie CP. Biogas production from municipal sewage sludge using ultrasound speeding digestion process. International Journal of Science and Engineering Investigations. 2013;2(15):109–118.

Chukwuneke JL, Sinebe JE, Ugwuegbu DC, Agulonu CC. Production by pyrolysis and analysis of bio-oil from mahogany wood (Swietenia macrophylla). British Journal of Applied Science & Technology. 2016;17(4):1–9.

Chukwuneke JL, Azaka OA, Chukwujike IC, Sinebe JE. Optimization of waste management system in Anambra State: Case study of Ifite-Awka. American Journal of Engineering Research. 2016;5(8):138–151.

Papurello D, Gandiglio M, Kafashan J, Lanzini A. Biogas purification: A comparison of adsorption performance in D4 siloxane removal between commercial activated carbons and waste wood-derived char using isotherm equations. Processes. 2019;7:774.
DOI: 10.3390/pr7100774

Obiora EA, Chinonso HA, Jeremiah LC, Paul CO. A mathematical model of a commercial thermophilic biogas plant. International Journal of Mechanical Engineering. 2012;40(10):343–349.

Lv Z, Feng L, Shao L, Kou W, Liu P, Dong X, Yu M, Wang J, Zhang D. The effect of digested manure on biogas productivity and microstructure evolution of corn stalks in anaerobic cofermentation. BioMed Research International. 2018;1–11.
DOI: 10.1155/2018/5214369

Mashudu M, Ashira R, Rasheed A, Mokhele M, Rosina M. Comparative assessment of bio-fertiliser quality of cow dung & anaerobic digestion effluent. Cogent Food & Agriculture. 2018;4:1-11.

Ge X, Matsumoto T, Keith L, Li Y. Biogas energy production from tropical biomass wastes by anaerobic digestion. Bioresource Technology. 2014;169:38–44.

Dhanalakshmi SV, Ramanujam RA. Biogas generation in a vegetable waste anaerobic digester: Analytical approach research. Recent Sciencies. 2012;3:41-47.

Zhang Q, Hu J, Lee DJ. Biogas from anaerobic digestion processes: Research updates. Renewable Energy. 2016;108-119.

Krishna N, Devi SS, Viswnath P, Deepak S, Sarada R. Anaerobic digestion of canteen wastes for biogas production: Process optimization. Process Biochemistry. 1991;26:1–5.

Mkiramweni LLN. The impact of biogas conversion technology for economic development: A case study in Kilimanjaro Region. International Scholarly Research Notices; 2012. Article ID: 424105.

Reza Alayi, Ali Shamel, Alibakhsh Kasaeian, Hossein Harasii, Majid Amani Topchlar. The role of biogas to sustainable development (aspects environmental, security and economic). Journal of Chemical and Pharmaceutical Research. 2016;8(4):112-118.

Sunita Baniya. Comparison of methane production from household waste using pond soil and horse dung as microbial inocula. University of Texas at Arlington. USA. 2016;1–103.

Selvankumar T, Sudhakar C, Govindaraju M, Selvan K, Aroulmoji V, Sivakumar N, Govarthanan M. Process optimization of biogas energy production from cow dung with alkali pre-treated coffee pulp. Biotech. 2017;7(254):1–8.

Shakira Ghazanfar, Nauman Khalid, Iftikhar Ahmed, Muhammad Imran. Probiotic yeast: Mode of action and its effects on ruminant nutrition. 2017;Chpt 8:179–202.

Cho SJ, Cho KM, Shin EC, Lim WJ, Hong SY, Byoung RC, Jung MK, Sun ML, Yong HK, Kim H, Yun HD. 16S rDNA analysis of bacterial diversity in three fractions of cow rumen. Journal of Microbiology and Biotechnology. 2006;16: 92-101.

Hartmann H, Angelidaki I, Ahring BK. Co-digestion of the organic fraction of municipal waste. IWA. 2002;181-200.

Safari Mahmood, Reza Abdi, Mehrdad Adi, Jalal Kafashan. Optimization of biogas productivity in lab-scale by response surface methodology. Renewable Energy; 2017.

Fernandaz A, Sanchez A, Font X. Anearobic co-digestion of a simulated organic fraction of municipal solid wastes and fats of animal and vegetable origin. Biochemical Engineering Journal. 2005;26:22-28.

Okolie NP, Onofade AK, Oladumonye MK, Adegunloye DV. Comparative study of commercial gas with biogas produced from co-digestion of corn cob, rice chaff, goat and dog dung. International Journal of Physical Science. 2018;13(6):98–105.

Grisel C, Laura P, Umapada P, Fortino B, Minerva R. Generation of biogas from coffee-pulp and cow-dung co-digestion: Infrared studies of postcombustion emission. Energy Conversion and Management. 2013;74:471–481.

Nwadike EC, Azaka OA, Okolie PC, Chukwuneke JL. Surface response optimization GCMS/FTIR analysis of biodiesel produced from cow-tallow and its blend with conventional diesel. Academic Journal of Science. 2017;7(2):345–356.

Su J, Shen F, Qiu M, Qi X. High-yield production of Levulinic acid from pretreated cow dung in dilute acid aqueous solution. Molecules. 2017;22(285):1–9.

Jin Wenyao, Xiaochen Xu, Fenglin Yang. Application of rumen microorganisms for enhancing biogas production of corn straw and livestock manure in a pilot-scale anaerobic digestion system: Performance and microbial community analysis. Energies; 2018.

APHA. Standard methods for the examination of water and wastewater (21st Ed.). Washington, DC: Author; 2005.

Pavia DL, Lampman GM, Kritz GS, Engel RG. Introduction to organic laboratory techniques: A microscale approach (4th Ed.). Cengage Learning, Brooks/Cole Publishing Co., St. Paul, MN. 2006;797–817. ISBN: 978- 049-5016-30-4.

Pinto O, Romero R, Carrier M, Appelt J, Segura C. Fast pyrolysis of tannins from pine bark as a renewable source of catechols. J. Anal. Appl. Pyrolysis. 2018;136:69–76.

Ofomatah AC, Obasi EE. Biogas optimization potentials of cow dung, pig dung and poultry droppings with sugar cane bagasse and water melon peel. FUW Trends in Science & Technology Journal. 2017;2(1A):267–270.